Figure 3.
Glucocorticoid metabolism in adipose tissues. 11β-HSD1 activity measured as velocity of formation of product following incubation of [3H]4-corticosterone with homogenates of adipose tissues from (A) Db/? control (open) or db/db mice (light striped); (B) Ob/? control (black) or ob/ob mice (dark striped). Abundance of mRNA for 11β-HSD1 in adipose tissue depots measured by real-time PCR (corrected for cyclophilin A as a housekeeping gene and presented as a percentage of respective control group) in (C) Db/? control or db/db mice; (D) Ob/? control or ob/ob mice. Abundance of mRNAs for 5α-R1 in adipose beds measured by real-time PCR (corrected for cyclophilin A as a housekeeping gene and presented as a percentage of respective control group) in (E) Db/? control or db/db mice; (F) Ob/? control or ob/ob mice. Subcut is s.c. adipose, Epi is epididymal, R/P is retro-peritoneal and Mes is mesenteric. Data are mean±s.e.m.; n=6–12/group; *P<0·05; **P<0·01.