Figure 2.

RalA induces filopodia in serum-starved Swiss 3T3 cells. Serum-starved, confluent Swiss 3T3 fibroblasts were fixed 15 min after injection with FITC-dextran alone (a), together with RalA (1.4 mg/ml) (b), V23RalA (0.4 mg/ml) (c), or coinjection with V23RalA (0.4 mg/ml) and N17Cdc42 (2.0 mg/ml) (d), V23RalA (0.7 mg/ml) and GST (0.8 mg/ml) (i), V23RalA (0.7 mg/ml) and GST-filamin 1 (2554–2647) (1.2 mg/ml) (j). Cells were fixed 5 min (e and f) or 90 min (g and h) after injection with V12 Cdc42 (0.46 mg/ml) (e and g), or coinjection with V12Cdc42 (0.46 mg/ml) and N28RalA (1.6 mg/ml) (f and h). Actin was localized by Texas red-X phalloidin. Injected cells were marked by FITC-labeled Dextran and indicated by arrows. (Scale bar represents 10 μm.)