Figure 4.

Distribution of actin in human melanoma cells. Serum-starved subconfluent filamin⁻¹-deficient M2 (a–c) or filamin⁻¹-repleted A7 (d) cells were injected with RhoA (1.0 mg/ml) (a), Rac1 (1.8 mg/ml) (b), or V12Cdc42 (1.8 mg/ml) (c and d). Cells were fixed at 180 (a), 150 (b), or 70 (c and d) min after injection and stained for actin filaments by using Texas red-X phalloidin. Injected cells were marked by FITC-labeled Dextran and indicated by arrows. (Scale bar represents 10 μm.)