Figure 5.
Distribution of actin and filamin 1 in human melanoma cells. Serum-starved subconfluent filamin-deficient M2 (a and b), filamin−1-repleted A7 (c–f), or M2 cells transfected with RalA-binding site deletion mutant filamin 1 [M2(δ CT112)] (g and h) were injected with V23RalA (1.6 mg/ml). Cells were fixed 90 min after injection and stained for actin filaments by using Texas red-X phalloidin (b, d, and h) or filamin 1 by using antifilamin antibody (f). Injected cells were marked by FITC-labeled Dextran (a, c, e, and g). (f) Accumulation of filamin 1 at the roots of filopods is indicated by arrows. (Scale bar represents 10 μm.)