TABLE 2.
PCR analysis of putative D1 deletions generated by imprecise P excision
| Primer seta | Genomic coordinatesb | Line 1A | Line 4A | Line 70-7 |
|---|---|---|---|---|
| pum 6925F + pum 7624R | 5,059,192–5,059,891 | + | + | + |
| pum 9494F + pum 9835R | 5,061,761–5,062,102 | + | + | + |
| D1 21F + D1 921R | 5,063,450–5,064,350 | + | − | + |
| D1 853F + D1 1699R | 5,064,282–5,065,128 | + | − | + |
| D1 1605F + D1 2305R | 5,065,034–5,065,734 | + | − | + |
| D1 2171F + D1 3285R | 5,065,600–5,065,914 | + | − | + |
| D1 2171F + D1 2965R | 5,065,600–5,066,394 | − | − | − |
| D1 3374F + D1 2965R | 5,066,003–5,066,394 | + | − | − |
| D1 3688F + D1 4320R | 5,066,317–5,066,949 | + | − | − |
| D1 3941F + D1 4320R | 5,066,570–5,066,949 | + | − | − |
The generation of a PCR product is indicated by a plus and the absence of a product by a minus.
a
The results of overlapping PCR amplifications that did not yield additional information are omitted.
b
The genomic coordinates of the amplified regions correspond to Flybase Release 5.1 of chromosome 3R. The insertion site for the D1EP473 element is 5,065,965–5,065,972 (8-bp duplication). The D1 gene coding region extends between 5,064,241 and 5,065,626 and the pum coding regions extend between 4,896,667 and 5,059,583.