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Comparison of GFP localization and FM4-64 staining. An S. cerevisiae vps4Δ mutant host expressing both Snf1-GFP and Snf4-Vps32 was stained with membrane dye FM4-64. The dye accumulates in endosome-derived class E compartments in vps4 mutants. DIC, GFP, and FM4-64 channels are shown. White arrows indicate regions of GFP and FM4-64 colocalization.
