TABLE 1.
Primer sequences
| Primer name | Sequence (5′–3′) |
|---|---|
| pRS314.GFP F | aagcttgatatcgaattcctgcagcccgggggatccactagttctagagcggccgccaccGGTCGACGGATCCCCGGGTT |
| pRS314.GFP R | acgacgttgtaaaacgacggccagtgaattgtaatacgactcactatagggcgaattggaTCGATGAATTCGAGCTCGTT |
| 314 cloning F | ggatccactagttctagagcggccgccacc |
| 314 cloning R | GTTAATTAACCCGGGGATCCGTCGACC |
| GFPtoSNF7 F | ACGCTGCAGGTCGACGGATCCCCGGGTTAATTAACtggtcatcactttttggttgg |
| GFPtoSNF7 R | TCATAAGAAATTCGCTTATTTAGAAGTGGCGCGCCtcaaagccccatttctgcttg |
| HIS5toLEU2 F | TACAGTTCTCACATCACATCCGAACATAAACAACCatgtctgcccctaagaagatc |
| HIS5toLEU2 R | CTTGAAAACAAGAATCTTTTTATTGTCAGTACTCTttaagcaaggattttcttaac |
| GFP.URA3 PGEMT F | GGTGGTGGTTCTAAAGGTGAAGAATTATT |
| GFP.URA3 PGEMT R | TCTAGAAGGACCACCTTTGATTG |
| CaSNF7 F | ACGCTGCAGGTCGACGGATCCCCGGGTTAATTAACTGGGGATATTTTTTTGGAGGA |
| CaSNF7 R | TCATAAGAAATTCGCTTATTTAGAAGTGGCGCGCCTCATAATCCCATTTCAGCTTG |
| CaHIS1 F | TACAGTTCTCACATCACATCCGAACATAAACAACCTGTGGAATTGTGAGCGGATA |
| CaHIS1 R | CTTGAAAACAAGAATCTTTTTATTGTCAGTACTTTCCCAGTCACGACGTT |
| CaPBS2 GFP F | GTTCAATCATTATTGAGAAACAAAGTGAAGGCTCCGGCATTACATAGAGGTGGTTTACAAAAAGTGAATAGAAGCTTTCTTAATAATCATGGTGGTGGTTCTAAAGGTGAAGAATTATT |
| CaPBS2 GFP R | GTGTTTGTGTTAGTTTGTTAGTTTGTTTATTTATTTGTTTGTTTCTATATAATA TACTGTTTATAATACAGCCCAATAACCTGGGCTTCATATTCATCTAGAAGGACCACCTTTGATTG |
| CaHOG1 pRSfus F | GGATCCACTAGTTCTAGAGCGGCCGCCACCTTTCCGTTAAAGTGTCCACTT |
| CaHOG1 pRSfus R | GTT AAT TAA CCC GGG GAT CCG TCG ACC AGC TCC GTT GGC GGA ATC CAA |
| CaSNF1 GFP F | CTAGATGAAGTTGGGTCATTCTCTGCTTATCCTTTCTTACATTTAGCTACTAGA TTAATTATGGAATTAGCCGTAAATAGTCAAAGTGGAGGTGGTGGTTCTAAAGGTGAAGAATTATT |
| CaSNF1 GFP R | GGCGTAAGAAATCCAAAAAATGGGTTGTGAATTTATCATACATATTACATATCTGCTGACATCCAATCTAAGCTAGTACTTACTTACTTTATTTCTAGAAGGACCACCTTTGATTG |
| CaSNF4 pRSfus F | GGATCCACTAGTTCTAGAGCGGCCGCCACCTGAATTGAATGTAAAAGAAGA |
| CaSNF4 pRSfus R | GTTAATTAACCCGGGGATCCGTCGACCATCTTCTCCAAATAATATGTA |