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. 2009 May;182(1):91–103. doi: 10.1534/genetics.108.099895

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Copyright © 2009 by the Genetics Society of America

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Figure 2.— — The presence of Rf9 causes a reduction in the linearized copies of the orf355/orf77 sequences. mtDNAs from ear shoots were digested with XhoI and gel blots were hybridized with an orf355-specific probe. VG and S are different subtypes of CMS-S. Nuclear genotype B37 carries recessive alleles for both rf3 and rf9. A619 carries the dominant Rf9 restorer, whereas Ky21 and Tr carry the dominant Rf3 restorer. (Lane 1) S-B37 × A619 (Rf9/rf9; rf3/rf3). (Lane 2) S-B37 × Ky21 (rf9/rf9; Rf3/rf3). (Lane 3) VG-A619 (Rf9/Rf9; rf3/rf3). (Lane 4) S-B37 × Tr (rf9/rf9; Rf3/rf3).