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. 2009 May 8;4(5):e5452. doi: 10.1371/journal.pone.0005452

Figure 2. Relative mRNA expression of steroidogenic enzymes in the chick CAM.

Figure 2

RT-qPCR analysis of mRNA coding for StAR, P450scc, P45017α, 3β-HSD and 17β-HSD on chick embryonic days 8 (n = 10), 10 (n = 17), 12 (n = 14), 14 (n = 10), 16 (n = 14), and 18 (n = 12). Data are reported as relative mRNA expression and represent mean normalized mRNA transcript number in copies/µL±SEM. 3β-HSD increased (F5, 68 = 89.282, p<0.0001) and 17β-HSD decreased (F5, 68 = 16.027, p<0.0001) significantly between embryonic day 8 and day 18.