Skip to main content
. 2009 Mar 7;4(5):e5466. doi: 10.1371/journal.pone.0005466

Figure 5. MAVS-induced apoptosis is independent of type I IFNs production.

Figure 5

(A) Three molecules (ΔRIG-I, MDA5 and MAVS) that activate the production of type I IFNs were introduced into HEK293T cells; only MAVS is able to induce apoptosis. (B) Anti-IFN-β antibody was added to the medium at the final concentration of 200 neutralization units/ml to prevent secreted IFN-β binding to IFNAR, this neutralization process did not inhibit MAVS-induced apoptosis. (C) SeV-induced apoptosis is IFN-I-independent. MEFs from both C57BL/6 wild type and IFNAR−/− mice were infected with rSeV-GFP. Cells were stained with Hoechst and PI 48 h post-infection. (D) The non-degradable form of IκB was co-expressed with different doses of MAVS, yet cell viability loss was not restored. (E) HEK293T cells were plated at both the lower chamber and upper chamber of the transwell plate separated by an insert filter membrane with 0.4 µm pore size. 1 µg of MAVS-containing plasmid was transfected into the cells in the lower chamber and all cells were harvested 48 h post-transfection for flow cytometry analysis.