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Nrp1 and VEGFR2 colocalized during uptake in response to sema3C. a, Mouse ECs fixed at the indicated time points during uptake in response to sema3C (250 ng/mL), labeled with antiNrp1 (red) and anti VEGFR2 (R2) (green). Scale bars: 10 µm (2 µm in the magnified subfields). b, Quantification of the time course of colocalization of Nrp1 with VEGFR2 in ECs treated with sema3C (250 ng/mL, open squares) or VEGF (50 ng/mL, filled squares) (n=3; mean±SD; P=0.003, P=0.03).
