Figure 6.
Nrp1 and plexin D1 colocalized during uptake in response to sema3C. a, Mouse ECs fixed at the indicated time points during uptake in response to sema3C (250 ng/mL), labeled with anti-Nrp1 (red) and anti-plexin D1 (D1) (green). Scale bars: 10 µm (2 µm the magnified subfields). b, Quantification of the time course of the colocalization of Nrp1 with plexin D1 (filled squares) and of Nrp1 with VEGFR2 (open squares, shown also in Figure 3b) in ECs treated with sema3C (250 ng/mL) (n=3; mean±SD; P=1×10−6, P=0.00015, P=0.0005).