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. 2009 May 7;4(5):e5471. doi: 10.1371/journal.pone.0005471

Figure 3. CTMP expression affects mitochondrial network organization.

Figure 3

(A) Confocal microscopy of CTMP expression in tetracycline inducible HeLa clones stably expressing pTer plasmid coding for a control short hairpin (Sh-cont) or a short hairpin directed against CTMP (Sh#1 and Sh#2). Cells were visualized by immunofluorescence after 5 days culture in the presence or absence of tetracycline (2 µg/mL), together with the MitoTracker Red. (B) Representative confocal pictures of mitochondrial shape in tetracycline-inducible HeLa clones cultured 5 days in the presence of tetracycline. Mitochondria were visualized with MitoTracker Red. (C) Morphological analysis of mitochondria shape in HeLa Tet-on clones treated as in (A) for 3, 4 or 5 days. For each experiment, at least 200 cells were counted in three distinct fields. Data are means±SEM, n = 2. The differences in mean values are statistically significant (Sh#2 minus tetracycline compared to Sh#2 plus tetracycline) as determined by 1-way ANOVA; ** P≤0.001.