Figure 4. CTMP is not required for mitochondrial fusion.

(A) Hela cells expressing mt-RFP and mt-GFP transfected with either siRNA#1 and #2 against CTMP or the siRNA control were fused in 50% PEG1500 for 60 s, washed and fixed at different times (4, 6 and 8 h). (B) Time-course of mitochondrial fusion in HeLa cells treated as in (A). Mitochondrial fusion was measured from 30 randomly selected polykaryons and classified as described in (A), n = 2.