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Western blot analysis of whole wild type and IFT mutant embryos at 72 hpf. Immunoreactivity against IFT52 (top row) were detected in wild type and mutants at 72 hpf, confirming the expression of these subunits. IFT88 immunoreactivity (middle row) was not seen in the ift88 mutants but continued to be expressed in other mutants as well as wild type. Acetylated tubulin (AT) served as a control (bottom row).
