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. Author manuscript; available in PMC: 2010 May 15.
Published in final edited form as: J Immunol Methods. 2009 Mar 9;344(1):15–25. doi: 10.1016/j.jim.2009.02.005

Figure 2. In absence of exogenously added accessory cells, PHA induces proliferation and cytokine secretion in IMACS-CD4+ but not in IMACS/FACS- CD4+T cells.

Figure 2

CD4+ T cells were purified by IMACS (IMACS) or by IMACS followed by FACS (IMACS/FACS). CD4+ T cells (105 cells/well) were stimulated with medium alone (white bars) or PHA (10 μg/ml, grey bars) in flat bottom 96 well plates. (A) IL-2 was quantified in cell-free culture supernatants (18h) by ELISA. (B) IFN-γ was quantified in cell-free culture supernatants (72h) by ELISA. (C) Proliferation was measured at 72 h by [3H] thymidine incorporation and results expressed as counts per minute (CPM). Mean values ± SEM of 5 experiments with separate donors are shown.