Figure 3. Effects of TRH on membrane current–voltage relationship of orexin neurones.

A, the voltage-clamp protocol used to obtain data in B. B, currents obtained in response to voltage steps using a ‘low Ca²⁺’ extracellular solution (see Methods). Grey bars (a and b) show where the steady-state values were measured to produce the plot in C. C, net current activated by TRH (b minus a), n= 4 cells.