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Deletion of TIM8 or TIM13 causes redistribution of Tim9p from the 70-kDa complex to the 300-kDa complex. Proteins solubilized from purified yeast mitochondria with 0.16% n-dodecylmaltoside were subjected to “blue native gel” electrophoresis (ref. 20; 6–16% acrylamide) and analyzed for Tim9p by immunoblotting and decoration with [125I]protein A. WT, parental strain; Δtim13, TIM13 deleted; Δtim8, TIM8 deleted; Δtim13Δtim8, TIM13 and TIM8 deleted.
