Fig. 6.

Anti-TGF-β antibodies inhibit the suppressive activity of AC-SPL cells in the LTS. WT mice were immunized with TNP–BSA 1 week after they received an intracameral injection of TNP–BSA. One week after immunizing, spleen cell suspensions were prepared. The cell suspensions were incubated for 30 min at 4°C. in PBS, PBS + 15 μg anti-TGF-β 1–3 antibodies or control MOPC 21 IgG1 antibody. After incubation, the cells ± antibodies or anti-TGF-β antibody only were injected into footpads of TNP–BSA-immunized recipients immediately after the footpad received an epicutaneous challenge with PCl. Swelling was measured 24 h later. Data represent the mean swelling ± SEM of six mice per group pooled from two experiments. *P < 0.01. NAIVE, non-immunized; IMM, immunized.