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a) Autoradiograph showing the TOP-53 dependent Top2-oligo release product in WT cells, which is absent in rad32mre11-D65N and ctp1∆. The control lane C shows the drug-independent Top2-oligo product in S. cerevisiae. This experiment was repeated 3 times. b) Density trace of the autoradiograph presented in panel a. In WT, the TOP-53 dependent release product is visible as a small peak left of the spontaneous drug-independent release product.
