Table 2.

Influence of time in culture on differentiation pattern of adult rat cervical pericentral canal spinal cord NSPCs: experimental outline

Group no.	Neurosphere age	Total time in culture
Group 1	Primary neurospheres	14 days post-isolation
		+ 7 days differentiation on Matrigel +1% FBS
		= 21 days total in vitro
Group 2	Secondary neurospheres	21 days post-isolation
		+ 7 days differentiation on Matrigel + 1% FBS
		= 28 days total in vitro
Group 3	Tertiary neurospheres	28 days post-isolation
		+ 7 days differentiation on Matrigel + 1% FBS
		= 35 days total in vitro

This table outlines the experimental protocol for studying the influence of time in culture on the differentiation pattern of adult rat cervical pericentral canal spinal cord NSPCs. A detailed breakdown of the neurosphere propagation and differentiation stages are provided to clarify the total number of days in vitro for primary, secondary, and tertiary neurospheres. NSPC, neural stem/precursor cell.