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G2 arrest in Ts-SKOV3 cells is associated with a decrease in cyclin B1/cdc2 kinase activity. (A) Cdc2 kinase activity, as measured using Histone H1 as a substrate, as a function of time at 32°C. Cdc2 activity decreases in Ts-SKOV3 (●) but not SKOV3 (○) at 32°C. (B) The decrease in cdc2 kinase activity is not a result of a change in cdc2 protein levels or cdc2 inhibitory tyrosine phosphorylation as determined by Western blotting.
