Figure 3.
G2 arrest in Ts-SKOV3 is associated with a decrease in cyclin B1 protein and mRNA. (A) Total cyclin B1 protein levels, as determined by Western blotting, decrease in Ts-SKOV3 cells at 32°C. There is no change in cyclin B1 protein in SKOV3 cells at 32°C. There is a small change in cyclin A protein levels in Ts-SKOV3 at 32°C. (B) Total cyclin B1 mRNA decrease as a function of time in Ts-SKOV3 cells grown at 32°C. Cdc2 mRNA levels are unchanged at 32°C. Glyceraldehyde-3-phosphate dehydrogenase is used as a loading control.