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. 1999 Mar 2;96(5):2147–2152. doi: 10.1073/pnas.96.5.2147

Figure 5.

Figure 5

p53 decreases the activity of the cyclin B1 promoter. (A) The activity of the cyclin B1 promoter (36) (1,050 bp 5′ of transcription initiation) transiently transfected into Ts-SKOV3 cells is markedly lower at 32°C (solid bars) than at 37°C (open bars) 24 and 48 hr after transfection. Promoter activity is not altered substantially in SKOV3 cells between 37°C (open bars) and 32°C (solid bars). (B) Wild-type p53 can suppress the activity of the cyclin B1 promoter in transient transfection assays in SKOV3, DP16, Saos-2, and A431 cells. Assays were performed at 37°C, and all data points are the mean of duplicate CAT measurements.