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. Author manuscript; available in PMC: 2009 Apr 30.
Published in final edited form as: Chem Phys Lett. 2008 Sep 1;462(1-3):6–9. doi: 10.1016/j.cplett.2008.07.043

Fig. 1.

Fig. 1

Circular dichroism data reflecting structural changes to SNAREs, both in suspension and in association with membrane. Structural changes, following the assembly and disassembly of the t-/v-SNARE complex is further shown. (A) CD spectra of purified full-length SNARE proteins in suspension and (B) in membrane-associated; their assembly and (NSF–ATP)-induced disassembly is demonstrated. (i) v-SNARE; (ii) t-SNAREs; (iii) t-/v-SNARE complex; (iv) t-/v-SNARE + NSF and (v) t-/v-SNARE + NSF + 2.5 mM ATP, is shown. CD spectra were recorded at 25 °C in 5 mM sodium phosphate buffer (pH 7.5), at a protein concentration of 10 μM. In each experiment, 30 scans were averaged per sample for enhanced signal to noise, and data were acquired on duplicate independent samples to ensure reproducibility.