Figure 7.

A, B, Retrograde tracing of inputs to GnRH neurons from leptin-responsive PMv cells in BIG mice. Female BIG mice were administered 5 mg/kg leptin i.p. for 1 h. Representative images show colocalization of the transsynaptic tracer BL (WGA-IR, green, cytoplasmic) around leptin-induced pSTAT3-IR (red, nuclear) in the PMv of BIG mice (A). No WGA-IR was detected in leptin-treated control mice (B). Insets show high magnification of colocalized BL and pSTAT3. Scale bar: 100 μm; 3v, third cerebral ventricle.