Figure 1.

Changes in mitochondrial Ca²⁺ (Ca²⁺_m) in astrocytes following OGD/REOX. (A) Mitotracker (green in a) and Rhod-2 (red in b) images were shown in NKCC1^+/+ astrocytes at the end of 2 h OGD and at 60 min REOX (c and d). Scale bar = 12.3 µm. (B) Average tracings of relative change in Rhod-2 fluorescence during 60 min REOX following 2 h OGD in NKCC1^+/+ and NKCC1^−/− astrocytes. Arrow: response of Rhod-2 fluorescence to FCCP. (C) Summary data. Data are means ± SEM; n = 4–5. * p < 0.05 vs. control; # p < 0.05 vs. NKCC1^+/+ OGD/REOX. Reprinted with permission from Kinter et al.⁸