Figure 2.
Pretreatment with PUFA in IEC-6 cells reduced PAF-induced TLR4 (□) and PAFR (■) expression. Data were presented as a relative change based on RT-PCR data compared with control IEC-6 cells. After pretreatment of AA (a), or DHA (b), TLR4 mRNA expressions in IEC-6 cells treated with PAF were significantly lower than those of untreated controls and treated with PAF alone (all p < 0.05). PUFA also inhibited PAFR mRNA expression induced by PAF. PAFR mRNA levels were significantly decreased in IEC-6 cells pretreated with AA or DHA compared with those treated with PAF alone (both p < 0.05). *p < 0.01 compared with untreated controls; **p < 0.05 compared with untreated controls.