Fig. 2.

Effect of prolonged hyperoxic exposure on the expression and phosphorylation of translation initiation and elongation factors. Proliferating human lung fibroblasts were incubated in either room air (control) or 95% O₂ for 72 h. Cell number was determined on duplicate wells, and the cells were lysed directly into lysis buffer. Protein extracts from equal cell numbers were subjected to immunoblotting on PVDF membranes (n = 4–8). Top: representative immunoblots. Bottom: histograms show the effect of hyperoxia on the phosphorylation of eIF2α and eIF4E corrected for the expression of β-actin at each time point. Values are expressed in arbitrary units. Columns represent means and bars denote SE. *P < 0.05 between control and 95% oxygen-treated cells.