Table 1.

Assessment of anti-EEV NAb responses by comet-reduction assay.

Group	Monkey	Comet Reduction Score^a

		Wk 0	Wk 4	Wk 13	Wk 30	Wk 48
rVV / rFPV	88M	0	+ +	+ + +	+ + +	+ + +
	90M	0	+	+ + +	+ + +	+ + +
	91M	0	+ +	+ + +	+ + +	+ + +
	92M	0	+ +	+ + +	+ +	+ +
	95M	0	+	+ +	+ +	+ +
	96M	0	+	+ + +	+ + +	+ +
rMVA / rFPV	80M	0	0	+ +	+	+
	81M	0	+	+ + +	+ +	+ +
	82M	0	0	+ +	+	+
	83M	0	0	+ +	+ +	+
	84M	0	0	+ +	+ +	+
	87M	0	+	+ + +	+ +	+ +
rMVA / rMVA	001M	0	+	+ + +	+ + +	+ + +
	002M	0	+	+ + +	+ + +	+ + +
	003M	0	+	+ +	+ + +	+ + +
	77M	0	0	+ +	+ + +	+ + +
	78M	0	+	+ +	+ + +	+ +
	996L	0	+	+ +	+ + +	+ +

VIG^b	Control	+ + +	-	-	-	-

Plasma samples from individual monkeys were isolated following Prime 1 (Wk 4), Prime 2 (Wk 13), Boost 1 (Wk 30), or Boost 2 (Wk 48) and tested in a comet reduction assay at a 1:50 dilution. Matched pre-immune plasma (Wk 0) served as a negative control. The degree of comet reduction was scored as follows: 0, no reduction; +, some; ++, intermediate; +++, complete.

VIG was used as a positive control at 1:500 dilution.