TABLE 2.
MICs for quinolones and fluoroquinolones and mutations in gyrA, gyrB, and parC detected in naturally and artificially induced resistant strains
Strain(s) | MIC (μg ml−1) for indicated antibioticb
|
Gene mutationa
|
||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
gyrA
|
gyrB
|
parC
|
||||||||||||||
Position
|
Codon change | aa change | Position
|
Codon change | aa change | Position
|
Codon change | aa change | ||||||||
NAL | OA | UB | CIP | nt | aa | nt | aa | nt | aa | |||||||
CG100 | 0.5 (S) | 0.125 (S) | 0.0625 (S) | 0.0078 (S) | ||||||||||||
FR1 | 16 (R) | 1 (S) | 0.25 (S) | 0.125 (S) | 248 | 83 | AGT→ATT | S→I | NC | NC | NC | NC | NC | NC | NC | NC |
FR2 | 16 (R) | 1 (S) | 0.25 (S) | 0.125 (S) | 248 | 83 | AGT→ATT | S→I | NC | NC | NC | NC | NC | NC | NC | NC |
CECT 4604 | 0.25 (S) | 0.0625 (S) | 0.0625 (S) | 0.0078 (S) | ||||||||||||
FR3 | 32 (R) | 2 (S) | 0.5 (S) | 0.125 (S) | 248 | 83 | AGT→ATT | S→I | NC | NC | NC | NC | NC | NC | NC | NC |
FR4 | 32 (R) | 2 (S) | 0.5 (S) | 0.125 (S) | 248 | 83 | AGT→ATT | S→I | NC | NC | NC | NC | NC | NC | NC | NC |
FR5 | 256 (R) | 16 (R) | 16 (R) | 1 (S) | 248 | 83 | AGT→ATT | S→I | 1156 | 386 | GCA→ACA | A→T | 254 | 85 | TCA→TTA | S→L |
1236 | 412 | CAG→CAC | Q→H | |||||||||||||
CECT 4602 | 128 (R) | 8 (R) | 64 (R) | 1 (S) | 248 | 83 | AGT→ATT | S→I | NC | NC | NC | NC | 254 | 85 | TCA→TTA | S→L |
CECT 4603, CECT 4606, CECT 4608, PD-5, PD-12, JE | 32 (R) | 2 (S) | <1 (S) | <1 (S) | 248 | 83 | AGT→ATT | S→I | NC | NC | NC | NC | NC | NC | NC | NC |
CECT 4862 | 64 (R) | 2 (S) | 2 (S) | <1 (S) | 249 | 83 | AGT→AGA | S→R | NC | NC | NC | NC | NC | NC | NC | NC |
A2, A4, A5, A6, A7, PD-1, PD-3 | 64-128 (R) | 2 (S) | 4 (S) | <1 (S) | 248 | 83 | AGT→ATT | S→I | NC | NC | NC | NC | 338 | 113 | GCA→GTA | A→V |
V1 | 128 (R) | 4 (S) | 4 (S) | <1 (S) | 248 | 83 | AGT→ATT | S→I | 1274 | 425 | GAG→GGG | E→G | NC | NC | NC | NC |
1314 | 438 | AAC→AAA | N→K |
Mutations in a nucleotide (nt) that gave rise to a codon change and to a change in amino acids (aa) are indicated. NC, no change detected.
The resistance (R) or sensitivity (S) against the antibiotic determined according to the Clinical and Laboratory Standards Institute and the European Committee for Antimicrobial Susceptibility Testing of the European Society of Clinical Microbiology and Infectious Diseases (9, 13) is indicated in parentheses.