TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli DH5α | DH1 F−Φ80ΔlacZΔM15 Δ(lacZYA-argF)U169 | N. T. Keen |
| X. fastidiosa | 7 | |
| A05 | Wild type | |
| DAC1984 | Gentamicin cassette replacing most of gacA ORF (ΔgacA::Gm) of X. fastidiosa A05 | This work |
| P. syringae pv. tomato | A. Chatterjee | |
| DC3000 | Wild type | |
| AC811 | Kmr; gacADC3000 derivative of DC3000 | A. Chatterjee |
| AC812 | Tcr Kmr; AC811 carrying pCPPgacAXf-Exp | This work |
| AC813 | Tcr Kmr; AC811 carrying plasmid pCPP47 | This work |
| E. amylovora | A. Chatterjee | |
| EC19 | Wild type | |
| EC191 | Gmr; gacA derivative of EC19 | A. Chatterjee |
| EC192 | Tcr Gmr; EC191 carrying pCPPgacAXf-Exp | This work |
| EC193 | Tcr Gmr; EC191 carrying plasmid pCPP47 | This work |
| Plasmids | ||
| pUC129 | Apr; cloning vector | New England Biolabs |
| pUCgacAXf-Exp | Apr; a fragment including the gacA promoter and ORF of X. fastidiosa (gacAXf-Exp) cloned into pUC129 | This work |
| pCPP47 | Tcr; broad-range plasmid | A. Chatterjee |
| pCPPgacAXf-Exp | Tcr; gacAXf-Exp cloned into pCPP47 constructed as gacAXf-Exp; expression plasmid | This work |
| pUC19841 | Apr; mutagenized PCR fragment of the flanking regions of gacA ORF of X. fastidiosa cloned into pUC129 | This work |
| pUC19842 | Apr Gmr; Gm cassette from pGEM-T-GM cloned into the AscI site of pUC19841 | This work |
| pGEM-T Easy | Apr; cloning vector | Promega |
| pBBR1MCS-5 | Gmr; broad-range plasmid | S. Lindow |
| pGEM-T-GM | Apr Gmr; Gm cassette from pBBR1MCS-5 cloned into pGEM-T | This work |