TABLE 2.
Variance component measuring indicated gene or transcript | No. of measured samplesa | Avg value ± SD |
---|---|---|
Cycle threshold | ||
An08g05910 | 230 | 24.08 ± 1.22 |
An02g04120 | 232 | 21.4 ± 0.98 |
An14g05050 | 236 | 25.69 ± 1.47 |
An08g06940 | 236 | 16.49 ± 0.57 |
An15g01860 | 233 | 24.83 ± 1.43 |
Kanamycin | 234 | 16.08 ± 0.49 |
xlnB | 236 | 22.62 ± 1.38 |
xlnD | 235 | 16.08 ± 0.82 |
Amplification efficiency | ||
An08g05910 | 230 | 1.70 ± 0.026 |
An02g04120 | 232 | 1.72 ± 0.044 |
An14g05050 | 236 | 1.68 ± 0.032 |
An08g06940 | 236 | 1.70 ± 0.024 |
An15g01860 | 233 | 1.71 ± 0.015 |
Kanamycin | 234 | 1.74 ± 0.028 |
xlnB | 236 | 1.73 ± 0.018 |
xlnD | 235 | 1.73 ± 0.027 |
Expression ratio | ||
An08g05910 | 226 | 1.06 ± 0.43 |
An02g04120 | 228 | 1.05 ± 0.43 |
An14g05050 | 232 | 1.02 ± 0.42 |
An08g06940 | 232 | 0.88 ± 0.32 |
An15g01860 | 229 | 2.45 ± 1.74 |
Kanamycin | 234 | 1.00 |
xlnB | 232 | 16.42 ± 9.35 |
xlnD | 231 | 137.73 ± 94.90 |
qPCR runs that did not meet quality control standards account for differences between the theoretical number of samples possible (240) and the actual number of measured samples.