TABLE 3.
Relative variance components estimatesa
| Variance component measuring indicated gene or transcript | Estimates by:
|
|||||
|---|---|---|---|---|---|---|
| Wk | Fermentor | Biomass sample | RNA sample | cDNA sample | qPCR measurement | |
| Cycle threshold | ||||||
| An08g05910 | 78.8 | 5.9 | 1.3 | 2.5 | 4.2 | 7.3 |
| An02g04120 | 66.2 | 1.9 | 0.0 | 0.3 | 11.5 | 20.1 |
| An14g05050 | 81.4 | 3.0 | 2.7 | 3.9 | 3.2 | 5.9 |
| An08g06940 | 68.1 | 5.5 | 1.0 | 14.1 | 8.3 | 2.9 |
| An15g01860 | 29.6 | 60.4 | 0.6 | 2.8 | 3.1 | 3.6 |
| Kanamycin | 67.5 | 0.0 | 0.0 | 18.1 | 10.1 | 4.3 |
| xlnB | 69.1 | 10.7 | 0.0 | 4.7 | 9.3 | 6.2 |
| xlnD | 4.8 | 19.4 | 2.9 | 47.7 | 8.2 | 17.0 |
| Amplification efficiency | ||||||
| An08g05910 | 68.9 | 2.1 | 2.8 | 0.0 | 1.1 | 25.1 |
| An02g04120 | 75.9 | 3.1 | 0.0 | 0.0 | 2.2 | 18.7 |
| An14g05050 | 74.7 | 0.1 | 0.0 | 0.0 | 0.3 | 24.9 |
| An08g06940 | 77.8 | 0.1 | 0.0 | 0.0 | 4.6 | 17.5 |
| An15g01860 | 30.1 | 5.3 | 0.0 | 1.5 | 8.2 | 54.8 |
| Kanamycin | 71.5 | 0.8 | 0.0 | 7.5 | 2.1 | 18.1 |
| xlnB | 31.2 | 6.2 | 7.1 | 0.0 | 11.3 | 44.3 |
| xlnD | 32.6 | 25.2 | 1.9 | 9.1 | 6.6 | 24.7 |
| Expression ratio | ||||||
| An08g05910 | 0.0 | 36.4 | 10.7 | 0.0 | 24.6 | 28.3 |
| An02g04120 | 2.1 | 28.9 | 0.0 | 4.3 | 21.5 | 43.2 |
| An14g05050 | 0.0 | 30.6 | 9.8 | 2.8 | 24.5 | 32.2 |
| An08g06940 | 0.3 | 58.5 | 2.3 | 12.7 | 19.8 | 6.3 |
| An15g01860 | 6.0 | 75.9 | 0.5 | 6.8 | 1.9 | 9.0 |
| Kanamycin | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| xlnB | 11.7 | 62.0 | 0.0 | 6.0 | 9.4 | 10.9 |
| xlnD | 0.0 | 80.7 | 1.6 | 3.6 | 11.5 | 2.6 |
a
Columns indicate the terms of the random model used to describe variance and correspond to the experimental procedure outlined in Fig. 2.