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. 2009 Feb 13;75(8):2446–2452. doi: 10.1128/AEM.02310-08

FIG. 1.

FIG. 1.

(a) Neisser-stained biomass from the Bendigo wastewater treatment plant showing Neisser-positive type 0092 filaments. Blue arrow = thicker type 0092; red arrow = thinner type 0092 filaments. (b) Confocal laser-scanning microscopy image of FISH-probed biomass from the Bendigo EBPR plant. Magenta cells are those responding to both GNSB941/CFX1223 mix (CY5) and CFX197 (CY3) FISH probes for Chloroflexi type 0092. Note the localized fluorescence signal. Blue cells are those responding to the GNSB941/CFX1223 mix probes alone. Note the absence of EUBmix FISH target sites; green cells are those responding only to the EUBmix (FLUOS) probes; light blue cells are those responding to EUBmix plus GNSB941/CFX1223 mix probes together. (c) Confocal laser-scanning microscopy image of FISH probed biomass from the Bendigo EBPR plant. Magenta cells are those responding to both GNSB941/CFX1223 mix (CY5) and CFX223 (CY3) FISH probes for Chloroflexi type 0092. Note again the localized fluorescence signal. Blue cells are those responding to the GNSB941/CFX1223 mix probes alone. Blue and magenta cells lack EUBmix FISH target sites; green cells are those responding only to the EUBmix (FLUOS) probes; light blue cells are those responding to EUBmix plus GNSB941/CFX1223 mix probes together.