Maximum-likelihood phylogenetic tree of the 16S rRNA gene sequences obtained in the present study and representatives from the Chloroflexi phylum. All sequences were at least 1,200 bp long except for AF495394, which was added in later using the quick-add function in ARB. Shading illustrates the coverage of each individual FISH probe. The scale bar corresponds to 0.1 substitutions per nucleotide position. Bootstrap values are calculated as a percentage of 1,000 analysis and are only indicated for values of ≥75%. Symbols: ○, bootstrap value of ≥75%; •, bootstrap value of ≥95%.