TABLE 2.
Results of FISH-based survey of Australian EBPR and non-EBPR full-scale plants for type 0092 using the CFX197 and CFX223 probes
Biomass samplea | Filament countb
|
||
---|---|---|---|
Neisser staining | CFX197 | CFX223 | |
Bendigo (VIC) | 6 | 5 | 3 |
Logan (QLD) | 4 | 4 | 3 |
Thorneside (QLD) | 2 | 2 | 1 |
Coolum (QLD) | 3 | 3 | 2 |
Nambour (QLD) | 3 | 3 | 2 |
Merrimac (QLD) | 5 | 3 | 2 |
Kyneton (VIC) | 1 | 1 | 0 |
Castlemaine (VIC) | 1 | 1 | 0 |
Watella (QLD) | 5 | 5 | 3 |
Mornington (VIC)c | 3 | 3 | 0 |
Daylesford (VIC)c | 2 | 2 | 0 |
Carrum (VIC) | 0 | 0 | 0 |
Maroochydore (QLD) | 2 | 2 | 1 |
Dalby (QLD) | 5 | 4 | 3 |
Morpeth (NSW) | 2 | 3 | 1 |
Orange (NSW) | 3 | 3 | 0 |
VIC, Victoria, Australia; QLD, Queensland, Australia; NSW, New South Wales, Australia.
Grading was as described in Jenkins et al. (22): 1, few; 2, some; 3, common; 4, very common; 5, abundant; and 6, excessive. The uptake of Neisser stain by floc EPS material meant the visualization of filaments within flocs was difficult. Therefore, estimations based only on staining may underestimate type 0092 abundance.
Non-EBPR plant.