TABLE 3.
FISH probes target site mismatches used to screen Chloroflexi 16S rRNA clone sequences to identify tentatively those derived from type 0092, as assessed by FISH analysis of Bendigo activated sludge biomass
| Probe(s) | 16S rRNA target sequencea | Reference |
|---|---|---|
| EUB338 I | ACTCCTACGGGAGGCAGC | 3 |
| Clones A26, A58, B1, and B45 | ATACCTACGGGTAGCAGC | |
| EUB338 II | ACACCTACGGGTGGCTGC | 14 |
| Clones A26, A58, B1, and B45 | ATACCTACGGGTAGCAGC | |
| EUB338 III | ACACCTACGGGTGGCAGC | 14 |
| Clones A26, A58, B1, B45 | ATACCTACGGGTAGCAGC | |
| CFX109 | ACGGCTGAGGAACACGTG | 8 |
| Clone A58 | ACGGGTGAGTAACATGTT | |
| Clones A26 and B45 | ACGGGTGAGTAACGCGTT | |
| Clone B1 | ACGGGTGAGTAATGCGTT | |
| CFX784 | GGGATTAGAGACCCCGGT | 8 |
| Clones A26, A58, B1, and B45 | GGGATTAGAAACCCCGGT |
a
Mismatches are underlined.