TABLE 2.
Oligonucleotides for gene disruption
| Gene | Direction | Sequencea |
|---|---|---|
| ΔdeoA | Forward | GTTTCAAACCGGCGGGCGGCGTGCGTACTGCGGAAGATGCGCAGAAATATGTGTAGGCTGGAGCTGCTTC |
| Reverse | GACGGGTCAGATTTGGCAGATTGAGCGGGCCTTTACGACCGTTATCAGCTCATATGAATATCCTCCTTAG | |
| Δtdk | Forward | ATGGCACAGCTATATTTCTACTATTCCGCAATGAATGCGGGTAAGTCTACGTGTAGGCTGGAGCTGCTTC |
| Reverse | TTAAGCGTGGCGATGCCTTTCCTGAATAGCCGTTAATGAGTCGACTTGTACATATGAATATCCTCCTTAG | |
| Δudp | Forward | TGATGTTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCGTGTAGGCTGGAGCTGCTTC |
| Reverse | GAATTACAGCAGACGACGCGCCGCTTCCACCACGATTTTCACCGCATGGCCATATGAATATCCTCCTTAG | |
| Δung | Forward | TGATGTTTTTCATCTCGGCCTCACTAAAAACGATTTACAAGGGGCTACGCGTGTAGGCTGGAGCTGCTTC |
| Reverse | GAATTACAGCAGACGACGCGCCGCTTCCACCACGATTTTCACCGCATGGCCATATGAATATCCTCCTTAG |
a
Underlined sequences are priming site sequences for template pKD3.