TABLE 3.
Effects on the expression of dut and the disruption of ung
| Strain | Cell growth (g/liter)a | Thymidine (mg/liter)a | Thymidine production per cell (mg/g DCW) | dUTP (nmol/g DCW)b | dTTP (nmol/g DCW)b | dUTP/dTTP ratio |
|---|---|---|---|---|---|---|
| BL21 | 12.5 ± 0.3c | NDd | 0 | 3.1 ± 0.9 | 630.2 ± 10.2 | 0.005 |
| BLdtu24 | 10.9 ± 0.3 | 459.0 ± 10.8 | 42.1 | 21.1 ± 3.2 | 621.3 ± 22.9 | 0.034 |
| BLdtu24 (dut) | 6.5 ± 1.5 | 376.2 ± 9.2 | 57.8 | 9.0 ± 0.2 | 1,317.8 ± 16.9 | 0.007 |
| BLdtug24 | 9.5 ± 0.5 | 550.7 ± 6.2 | 58.0 | 18.5 ± 2.3 | 441.4 ± 2.1 | 0.042 |
a
These data were obtained from flask cultures for 24 h.
b
Cultures were harvested at a mid-log phase.
c
The standard deviations were calculated by using standard propagation of error methods.
d
ND, not detected.