TABLE 1.
S. cerevisiae strains and plasmids used in this study
| Strain or plasmid | Relevant genotype/phenotype | Source or reference |
|---|---|---|
| S. cerevisiae strains | ||
| CEN.PK2-1C | MATaleu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2-8cSUC2 | K. D. Entian, Frankfurt, Germany |
| MKY9 | (MATaleu2-3,112 ura3-52 trp1-289 his3-Δ1MAL2-8cSUC2 PromTKL1::loxP-Prom-vkHXT7 PromRPE1::loxP-Prom-vkHXT7 PromRKI1::loxP-Prom-vkHXT7 PromGAL2::loxP-Prom-vkHXT7 PromXKS1::loxP-Prom-vkHXT7) + unknown beneficial mutations for pentose growth | Boles lab stock |
| BarraGrande | Industrial strain for bioethanol production | Brazilian ethanol plant |
| BWY10Xyl | BarraGrande with plasmid YEp-opt.XI-Clos-K evolved on xylose growth | This work |
| Plasmids | ||
| p426H7 | URA3 | 11 |
| YEp-XI-Agro | pHXT7; xylA from A. tumefaciens; tCYC1 URA3 | This work |
| YEp-XI-Arab | pHXT7; xylA from A. tumefaciens; tCYC1 URA3 | This work |
| YEp-XI-BaLi | pHXT7; xylA from B. licheniformis; tCYC1 URA3 | This work |
| YEp-XI-Burk | pHXT7; xylA from B. xenovorans; tCYC1 URA3 | This work |
| YEp-XI-Clos | pHXT7; xylA from C. phytofermentans; tCYC1 URA3 | This work |
| YEp-opt.XI-Clos | pHXT7; codon-optimized xylA from C. phytofermentans; tCYC1 URA3 | This work |
| YEp-XI-Lacto | pHXT7; xylA from L. pentosus; tCYC1 URA3 | This work |
| YEp-opt.XI-Piro | pHXT7; codon-optimized xylA from Piromyces sp. strain E2; tCYC1 URA3 | Boles lab stock |
| YEp-XI-Pseudo | pHXT7; xylA from Pseudomonas syringae; tCYC1 URA3 | This work |
| YEp-XI-Robi | pHXT7; xylA from R. biformata; tCYC1 URA3 | This work |
| YEp-XI-Saccha | pHXT7; xylA from S. degradans; tCYC1 URA3 | This work |
| YEp-XI-Salmo | pHXT7; xylA from S. enterica serovar Typhimurium; tCYC1 URA3 | This work |
| YEp-XI-Staph | pHXT7; xylA from S. xylosus; tCYC1 URA3 | This work |
| YEp-XI-Xantho | pHXT7; xylA from X. campestris; tCYC1 URA3 | This work |
| YEp-opt.XI-Clos-K | pHXT7; codon-optimized xylA from C. phytofermentans; tCYC1 URA3 loxP-kanMX-loxP FAA2 locus | This work |