TABLE 2.
AAB species monitoring from laboratory scale (stage 1) to factory scale (stages 2 and 3) as detected by PCR/DGGE and 16S rRNA gene sequencing
| Acetification stage | Days | Speciesa | 16S sequence accession no.b | Size (bp) | Species name, accession no.c | % Sequence similarity |
|---|---|---|---|---|---|---|
| 1 | 30 | A. pasteurianus | FM177227 | 1,410 | A. pasteurianus, DQ523493.1 | 100 |
| 2 | 60 | A. pasteurianus | FM177228 | 1,410 | A. pasteurianus, DQ523493.1 | 100 |
| 120 | A. pasteurianus/G. europaeusd | |||||
| 3 | 7 | G. europaeus | FM177229 | 1,451 | G. europaeus, EU096233.1 | 99 |
| 14 | G. europaeus | FM177230 | 1,450 | G. europaeus, EU096233.1 | 100 | |
| 21 | G. europaeus | FM177231 | 1,450 | G. europaeus, EU096233.1 | 100 | |
| 28 | G. europaeus | FM177232 | 1,450 | G. europaeus, EU096233.1 | 100 |
a
PCR/DGGE species designation obtained by clustering AAB species according to 16S rRNA/DGGE analysis (4). Bands of stage 2 (at 60 days) and stage 3 (at 28 days) were excised and sequenced.
b
Accession number obtained in this study.
c
Accession number of sequence of closest relative.
d
A mixed sequence chromatogram was obtained.