TABLE 2.
Acetification stage | Days | Speciesa | 16S sequence accession no.b | Size (bp) | Species name, accession no.c | % Sequence similarity |
---|---|---|---|---|---|---|
1 | 30 | A. pasteurianus | FM177227 | 1,410 | A. pasteurianus, DQ523493.1 | 100 |
2 | 60 | A. pasteurianus | FM177228 | 1,410 | A. pasteurianus, DQ523493.1 | 100 |
120 | A. pasteurianus/G. europaeusd | |||||
3 | 7 | G. europaeus | FM177229 | 1,451 | G. europaeus, EU096233.1 | 99 |
14 | G. europaeus | FM177230 | 1,450 | G. europaeus, EU096233.1 | 100 | |
21 | G. europaeus | FM177231 | 1,450 | G. europaeus, EU096233.1 | 100 | |
28 | G. europaeus | FM177232 | 1,450 | G. europaeus, EU096233.1 | 100 |
PCR/DGGE species designation obtained by clustering AAB species according to 16S rRNA/DGGE analysis (4). Bands of stage 2 (at 60 days) and stage 3 (at 28 days) were excised and sequenced.
Accession number obtained in this study.
Accession number of sequence of closest relative.
A mixed sequence chromatogram was obtained.