Immunostaining of individual immune cells in serial sections of a single islet from a patient with recent-onset type 1 diabetes (T1D). The micrographs reveal the extent of endocrine cell immunostaining and the immune cell complement in serial sections of one representative islet. Sections were stained with anti-sera raised against glucagon (a), insulin (b), CD45 (c), CD3 (d), CD4 (e), CD8 (f), CD20 (g) and CD68 (h) respectively. All images were taken at ×200 magnification.