Fig 3.

Analysis of complement sensitivity and membrane-bound complement regulatory protein (mCRP) level of SKOV3-multi-drug-resistant (MDR) cells. (a) Susceptibility to complement-mediated lysis of SKOV3 (black bars), SKOV3-MDR (grey bars) and SKOV3-MDR/2 (open bars) in ⁵¹Cr-release assay. Results are presented as mean % lysis ± standard deviation (s.d.), adjusted to lysis of SKOV3 parental cells (set as 100%; triplicates of n = six independent experiments). Control = MDR cells without P-glycoprotein (P-gp) blockade, +Vera and +cyclosporine A (CsA) = MDR cells incubated with verapamil (10 µg/ml) or CsA (1 µM), respectively, to block P-gp. (b) Expression of membrane-bound complement regulators on SKOV3 (black bars) and SKOV3-MDR/2 (open bars) cells (after long-term vincristine exposure). Results are presented as mean mCRP copies/cell ± s.d. (n = four independent experiments). **P < 0·01; ***P < 0·001.