Table 1 Conditions for polymerase chain reaction and restriction fragment length polymorphism.
| IFNγ T+874A polymorphism | IL12 GC/CTCTAA polymorphism | |
|---|---|---|
| Forward primer | 5′‐TTC TTA CAA CAC AAA ATC AAG TC‐3′ | 5′‐TGT TCT AAT GTG GGG GCC ACG‐3′ |
| Reverse primer | 5′‐AGT ATT CCC AAA AGG CTT ATG T‐3′ | 5′‐CTG TTT GTC AGC AGA CCT TCC T‐3′ |
| Denaturation temperature (20 s) | 94°C | 94°C |
| Annealing temperature (20 s) | 50°C | 55°C |
| Extension temperature (30 s) | 72°C | 72°C |
| Restriction enzyme | 8 U of Alw26* | 10 U of TaiI* |
| Overnight restriction, temperature | 37°C | 65°C |
| Product size | 340/26 bp A allele | 205/22 bp CTCTAA allele |
| 366 bp T allele | 223 bp GC allele |
IFN, interferon; IL, interleukin
* Provided by New England Biolabs, Beverly, Madison, USA