Figure 4.

hLhx2 correctly regulates ap target genes in Drosophila wing imaginal discs. Panels show β-galactosidase or Ser immunodetections following ectopic ap or hLhx2 expression in third instar larvae wing discs. The wild-type pattern of the fng-lacZ (A), Ser (D), vg-lacZ (G), and wg-lacZ (J) markers are depicted at the top. The dpp-GAL4 driver (M) was used to direct expression of the indicated UAS transgenes along the antero-posterior axis. Note that in all cases wing discs coexpressing dpp-GAL4 and either UAS:ap (B, E, H, K) or UAS:hLhx2 (C, F, I, L) exhibit ectopic activation of the molecular markers within the ventral compartment. Arrowhead indicates the wild-type expression of fng in the ventral compartment. Arrows point to the sites of ectopic expression.