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. 2009 Apr 14;10:159. doi: 10.1186/1471-2164-10-159

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Copyright © 2009 Giardina et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Agarose gel (1.0%) analysis of digested DNA samples. Lanes M are the size markers (50 bp ladder), lane 1 undigested genomic DNA, lanes 2–4 the digestion stopped after 30, 60 and 180 s, respectively. We decided to type samples digested for 180s, since they did not show evidence of partially-digested DNA fragments.