Table 1.
| Primer | Sequence [5’to 3’] | 5’ Position [bp] |
|---|---|---|
| HIVshortF | CCTGGGAGCTCTCTGGCTAA | 29 |
| HIVshortR | AACAGACGGGCACACACTACTTT | 119 |
| 3kb-F | CAATGACATACAGAAATTAGTGGGAAA | 2868 |
| 3kb-R | CTTTGGTTCCCCTAAGAAGTTTACAT | 2958 |
| 7kb-F | TGGCAGGAAGTAGGAAAAGCA | 7040 |
| 7kb-R | AGCAGCCCAGTAATATTTGATGAAC | 7120 |
| Luc-F | AGGTGGCTCCCGCTGAA | *9.6 kb |
| Luc-R | ACACCTGCGTCGAAGATGTTG | *9.7 kb |
| RPS9-F | GACGGGGAAGCGGAGCCAACA | **308 |
| RPS9-R1 | GCTTCAGCTCTTGGTCGAGACGAG | **418 |
| RPS9-R2 | GCGAGCGTGGTGGATGGACTTG | **5579 |
*
Primer locations are based on the luciferase gene sequence in Promega’s pGL-3 basic vector, forward primer at 1343 bp and reverse primer 1406 bp. The luciferase reporter gene is located in the nef gene of the HIV pNL4-3.LUC.R-E- genome at 8.3 kb.
**
Primer locations are based on Ensembl Gene ID: ENSG00000170889.