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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Mol Cell Cardiol. 2008 Oct 17;46(2):278–284. doi: 10.1016/j.yjmcc.2008.09.713

Figure 5. Ethanol treatment decreases activation of MAPK pathway signaling molecules.

Figure 5

Mitochondrial protein was isolated from the left ventricles of animals that were administered 0.5 g/kg of ethanol 60 minutes prior to LAD occlusion (Et) and compared to animals that were not treated with ethanol (C) and animals which did not undergo LAD occlusion (N). (a) Following homogenization, cardiac total lysate from the left ventricle was analyzed by Western blot to determine the phosphorylation levels of JNK1/2 (p-p46 and p-p54), and Erk1/2 (p-p44 and p-p42). Data from two separate groups of experiments are shown. (B) Quantification of the data in panel a was done using NIH Image-J software, expressed as % control and significance was determined using a 2 way t-test.