Figure 7.

Effect of inhibiting functional cystic fibrosis transmembrane regulator gene (CFTR) expression by antisense CFTR oligonucleotides (A) or by overexpressing mutated CFTR (B) on sarcoendoplasmic reticulum Ca²⁺-ATPase (SERCA)2 expression. Cell lysates were prepared from polarized cultures of 16HBEo- cell line stably transfected with sense (S) and antisense (AS) CFTR oligonucleotide and analyzed for SERCA2 expression by Western blot. The bars represent means of data. *Significant difference (P < 0.05) from control (16HBE-S) cells. The experiment was repeated four times. (B) Results of SERCA2 expression analysis in lysates obtained from control and adenovirally transduced minimally transformed primary human bronchial epithelial cells (UNCN3T) grown on collagen-coated culture dishes. The bars represent means of data. *Significant difference (P < 0.05) from control (LacZ) cells. The experiment was repeated three times (n = 3 per condition).